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American LabRats! Comment on OMB Regulation for Federal Financial Assistance -- or lose your research funding.
Projects must -- quote -- "demonstrably advance the president's policy priorities." And, in a major change, political appointees, not necessarily subject matter experts, would be required to give the final OK before money is awarded.
Also see this page on comment formatting requirements.
PDB101: Molecule of the Month: Nucleosome
Histones help pack DNA, but they can be modified in a lot of ways this article doesn't talk about: acetylation, methylation, phosphorylation, and ubiquitination. They're definitely more complex than they initially seem.
https://pdb101.rcsb.org/motm/7Open linkView original on mander.xyz131
Comments7China has research jobs.
Just got out of an initial interview with a Chinese lab. I was nervous at even the thought of moving that far, but they showed more interest in hiring me than any American lab (probably because Americans have no money for science). I'm setting up a time to present my research to them.
It's been really hard to find work, so while I'm nervous at the prospect of going somewhere so (supposedly) different, I hope this goes well.
Updated transient gene expression protocol in Expi293F cells using PEI
Still Expensive, but much cheaper Mammalian Expression Protocol. Good for Advanced DIY and Start Up Labs.
https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1661193/fullOpen linkView original on mander.xyz109
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Comments8Protein Design with Foundry
Does anyone have thoughts on how to characterize binding affinity of designed binders on the cheap? I was thinking maybe florescently labeled condensates could be used by checking the condensation transition and comparing it to known binders, but this conflates a lot of variables together so I'm not sure if that's a workable idea.
https://github.com/RosettaCommons/foundry/Open linkView original on mander.xyz